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antibodies against cd86 (Bioss)

Name: antibodies against cd86
Brand: Bioss
Rating: 95 (108 reviews)

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Bioss antibodies against cd86

The ratio of <t>CD86</t> + M1 macrophages is positively correlated with prostate tissue inflammation in patients. (a) Representative H&E images depicting the evaluation of inflammation grades in prostate tissue. (b , c) Representative IHC images of CD86 + M1 macrophages and CD206 + M2 macrophages in prostate tissue of patients with mild, moderate, and severe inflammation, respectively. Scale bars: 50 μm. (d) Quantitative analyses of CD86 + M1 macrophages, CD206 + M2 macrophages, and the M1/M2 ratio in prostate tissue. CD86 + M1 and CD206 + M2 cells were counted per high-power field (HPF) at × 200 magnification. (e) Spearman’s rank correlation analysis between inflammation scores and CD86 + cell counts, CD206 + cell counts, or M1/M2 ratios. Data are shown as the mean ± SEM ( n = 9 mild, 11 moderate, 6 severe) and were analyzed using a two-tailed unpaired Student’s t -test. * p < 0.05, ** p < 0.01, and *** p < 0.001 vs . the mild group.

Antibodies Against Cd86, supplied by Bioss, used in various techniques. Bioz Stars score: 95/100, based on 108 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 95 stars, based on 108 article reviews

antibodies against cd86 - by Bioz Stars, 2026-02

95/100 stars

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1) Product Images from "TENS alleviates CP/CPPS-related inflammation and pain by modulating Kir2.1-dependent macrophage polarization"

Article Title: TENS alleviates CP/CPPS-related inflammation and pain by modulating Kir2.1-dependent macrophage polarization

Journal: Frontiers in Immunology

doi: 10.3389/fimmu.2025.1683500

The ratio of CD86 + M1 macrophages is positively correlated with prostate tissue inflammation in patients. (a) Representative H&E images depicting the evaluation of inflammation grades in prostate tissue. (b , c) Representative IHC images of CD86 + M1 macrophages and CD206 + M2 macrophages in prostate tissue of patients with mild, moderate, and severe inflammation, respectively. Scale bars: 50 μm. (d) Quantitative analyses of CD86 + M1 macrophages, CD206 + M2 macrophages, and the M1/M2 ratio in prostate tissue. CD86 + M1 and CD206 + M2 cells were counted per high-power field (HPF) at × 200 magnification. (e) Spearman’s rank correlation analysis between inflammation scores and CD86 + cell counts, CD206 + cell counts, or M1/M2 ratios. Data are shown as the mean ± SEM ( n = 9 mild, 11 moderate, 6 severe) and were analyzed using a two-tailed unpaired Student’s t -test. * p < 0.05, ** p < 0.01, and *** p < 0.001 vs . the mild group.

Figure Legend Snippet: The ratio of CD86 + M1 macrophages is positively correlated with prostate tissue inflammation in patients. (a) Representative H&E images depicting the evaluation of inflammation grades in prostate tissue. (b , c) Representative IHC images of CD86 + M1 macrophages and CD206 + M2 macrophages in prostate tissue of patients with mild, moderate, and severe inflammation, respectively. Scale bars: 50 μm. (d) Quantitative analyses of CD86 + M1 macrophages, CD206 + M2 macrophages, and the M1/M2 ratio in prostate tissue. CD86 + M1 and CD206 + M2 cells were counted per high-power field (HPF) at × 200 magnification. (e) Spearman’s rank correlation analysis between inflammation scores and CD86 + cell counts, CD206 + cell counts, or M1/M2 ratios. Data are shown as the mean ± SEM ( n = 9 mild, 11 moderate, 6 severe) and were analyzed using a two-tailed unpaired Student’s t -test. * p < 0.05, ** p < 0.01, and *** p < 0.001 vs . the mild group.

Techniques Used: Two Tailed Test

TENS induced M2 polarization of macrophages in prostate tissue. (a) Representative IHC images of CD86 + M1 macrophages and CD206 + M2 macrophages in rat prostate tissue. Scale bars: 50 μm. (b) Quantitative analyses of CD86 + M1 macrophages, CD206 + M2 macrophages, and the M1/M2 ratio in prostate tissue. CD86 + M1 and CD206 + M2 cells were counted per high-power field (HPF) at × 200 magnification. Data are shown as the mean ± SEM and were analyzed using one-way ANOVA followed by Tukey’s post-hoc test. # p < 0.05 and ## p < 0.01 vs . the control group. * p < 0.05 and ** p < 0.01 vs . the EAP group ( n = 4 per group).

Figure Legend Snippet: TENS induced M2 polarization of macrophages in prostate tissue. (a) Representative IHC images of CD86 + M1 macrophages and CD206 + M2 macrophages in rat prostate tissue. Scale bars: 50 μm. (b) Quantitative analyses of CD86 + M1 macrophages, CD206 + M2 macrophages, and the M1/M2 ratio in prostate tissue. CD86 + M1 and CD206 + M2 cells were counted per high-power field (HPF) at × 200 magnification. Data are shown as the mean ± SEM and were analyzed using one-way ANOVA followed by Tukey’s post-hoc test. # p < 0.05 and ## p < 0.01 vs . the control group. * p < 0.05 and ** p < 0.01 vs . the EAP group ( n = 4 per group).

Techniques Used: Control

Electrical stimulation promoted the repolarization of M1 macrophages into M2 macrophages. (a) Cell viability after ES was assessed using the CCK8 assay. (b) Flow cytometry analysis of CD86 + M1 and CD206 + M2 macrophages in control, LPS-treated, and ES-treated (0.1 V/cm, 0.25 V/cm) RAW264.7 cells. MFI, mean fluorescence intensity. (c) Reactive oxygen species (ROS) detection in control, LPS-treated, and ES-treated (0.1 V/cm, 0.25 V/cm) RAW264.7 cells. (d) Western blot analysis of M1 markers (CD86, iNOS, TLR4) and M2 markers (CD206, CD163, CD209) in control, LPS-stimulated, and LPS + ES-treated (0.25 V/cm) RAW264.7 cells. (e) Quantitative analyses of macrophage phenotype marker expression. (f) ELISA analyses of TNF-α, IL-1β, IL-6, and IL-10 secretion in macrophage culture supernatants. Data are shown as the mean ± SEM and were analyzed using one-way ANOVA followed by Tukey’s post-hoc test . ## p < 0.01 and ### p < 0.001 vs . the control group. * p < 0.05, ** p < 0.01, and *** p < 0.001 vs . the LPS group ( n = 3 per group).

Figure Legend Snippet: Electrical stimulation promoted the repolarization of M1 macrophages into M2 macrophages. (a) Cell viability after ES was assessed using the CCK8 assay. (b) Flow cytometry analysis of CD86 + M1 and CD206 + M2 macrophages in control, LPS-treated, and ES-treated (0.1 V/cm, 0.25 V/cm) RAW264.7 cells. MFI, mean fluorescence intensity. (c) Reactive oxygen species (ROS) detection in control, LPS-treated, and ES-treated (0.1 V/cm, 0.25 V/cm) RAW264.7 cells. (d) Western blot analysis of M1 markers (CD86, iNOS, TLR4) and M2 markers (CD206, CD163, CD209) in control, LPS-stimulated, and LPS + ES-treated (0.25 V/cm) RAW264.7 cells. (e) Quantitative analyses of macrophage phenotype marker expression. (f) ELISA analyses of TNF-α, IL-1β, IL-6, and IL-10 secretion in macrophage culture supernatants. Data are shown as the mean ± SEM and were analyzed using one-way ANOVA followed by Tukey’s post-hoc test . ## p < 0.01 and ### p < 0.001 vs . the control group. * p < 0.05, ** p < 0.01, and *** p < 0.001 vs . the LPS group ( n = 3 per group).

Techniques Used: CCK-8 Assay, Flow Cytometry, Control, Fluorescence, Western Blot, Marker, Expressing, Enzyme-linked Immunosorbent Assay

Repolarization mechanism of ES effect in macrophages. (a) GO classification of DEPs between the LPS and ES groups related to cellular component, molecular function, and biological process categories. (b) Heatmap of differentially clustered ion transmembrane transporter activity. The color scale represents the Z -score of normalized protein abundance; red indicates upregulation, and blue indicates downregulation. (c) Representative WB images of Kir2.1 and TRPV2 expression. (d) Quantitative analyses of Kir2.1 and TRPV2 marker expression. (e) Representative IHC images of Kir2.1 in rat prostate tissue. Scale bars: 50 μm. (f) Quantitative analyses of CD86 + and CD206 + expression in macrophages measured by flow cytometry. (g) Immunofluorescence staining images of cellular membrane potential in macrophages (scale bars: 20 μm). (h) Quantitative analysis of cellular membrane potential in macrophages measured by flow cytometry. (i) Immunofluorescence staining images of intracellular Ca 2+ concentration in macrophages (scale bars: 20 μm). (j) Quantitative analysis of intracellular Ca 2+ concentration in macrophages measured by flow cytometry. MFI, mean fluorescence intensity. (k) Representative WB images of NF-κB/STAT1/STAT6 signaling pathway expression. (l) Quantitative analyses of NF-κB/STAT1/STAT6 signaling pathway expression. Data are shown as the mean ± SEM and were analyzed using one-way ANOVA followed by Tukey’s post-hoc test. ## p < 0.01 and ### p < 0.001 vs . the LPS group. * p < 0.05, ** p < 0.01, and *** p < 0.001 vs . the ES group ( n = 3 per group).

Figure Legend Snippet: Repolarization mechanism of ES effect in macrophages. (a) GO classification of DEPs between the LPS and ES groups related to cellular component, molecular function, and biological process categories. (b) Heatmap of differentially clustered ion transmembrane transporter activity. The color scale represents the Z -score of normalized protein abundance; red indicates upregulation, and blue indicates downregulation. (c) Representative WB images of Kir2.1 and TRPV2 expression. (d) Quantitative analyses of Kir2.1 and TRPV2 marker expression. (e) Representative IHC images of Kir2.1 in rat prostate tissue. Scale bars: 50 μm. (f) Quantitative analyses of CD86 + and CD206 + expression in macrophages measured by flow cytometry. (g) Immunofluorescence staining images of cellular membrane potential in macrophages (scale bars: 20 μm). (h) Quantitative analysis of cellular membrane potential in macrophages measured by flow cytometry. (i) Immunofluorescence staining images of intracellular Ca 2+ concentration in macrophages (scale bars: 20 μm). (j) Quantitative analysis of intracellular Ca 2+ concentration in macrophages measured by flow cytometry. MFI, mean fluorescence intensity. (k) Representative WB images of NF-κB/STAT1/STAT6 signaling pathway expression. (l) Quantitative analyses of NF-κB/STAT1/STAT6 signaling pathway expression. Data are shown as the mean ± SEM and were analyzed using one-way ANOVA followed by Tukey’s post-hoc test. ## p < 0.01 and ### p < 0.001 vs . the LPS group. * p < 0.05, ** p < 0.01, and *** p < 0.001 vs . the ES group ( n = 3 per group).

Techniques Used: Activity Assay, Quantitative Proteomics, Expressing, Marker, Flow Cytometry, Immunofluorescence, Staining, Membrane, Concentration Assay, Fluorescence

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Journal: Frontiers in Immunology

Article Title: TENS alleviates CP/CPPS-related inflammation and pain by modulating Kir2.1-dependent macrophage polarization

doi: 10.3389/fimmu.2025.1683500

Figure Lengend Snippet: The ratio of CD86 + M1 macrophages is positively correlated with prostate tissue inflammation in patients. (a) Representative H&E images depicting the evaluation of inflammation grades in prostate tissue. (b , c) Representative IHC images of CD86 + M1 macrophages and CD206 + M2 macrophages in prostate tissue of patients with mild, moderate, and severe inflammation, respectively. Scale bars: 50 μm. (d) Quantitative analyses of CD86 + M1 macrophages, CD206 + M2 macrophages, and the M1/M2 ratio in prostate tissue. CD86 + M1 and CD206 + M2 cells were counted per high-power field (HPF) at × 200 magnification. (e) Spearman’s rank correlation analysis between inflammation scores and CD86 + cell counts, CD206 + cell counts, or M1/M2 ratios. Data are shown as the mean ± SEM ( n = 9 mild, 11 moderate, 6 severe) and were analyzed using a two-tailed unpaired Student’s t -test. * p < 0.05, ** p < 0.01, and *** p < 0.001 vs . the mild group.

Article Snippet: Sections were incubated overnight at 4°C with primary antibodies against CD86 (bs-1035R, Bioss, Beijing, China), CD206 (18704-1-AP, Proteintech, Wuhan, China), TNF-α ( GB115701 , Servicebio, Wuhan, China), IL-1β (GB11113, Servicebio, Wuhan, China), IL-6 (GB11117, Servicebio, Wuhan, China), Cyclooxygenase-2 (COX-2) (Abcam, ab179800, Cambridge, UK), substance P (SP; Proteintech, Wuhan, China, 13839-1-AP), or Kir2.1 (19965-1-AP, Proteintech, Wuhan, China).

Techniques: Two Tailed Test

Journal: Frontiers in Immunology

Article Title: TENS alleviates CP/CPPS-related inflammation and pain by modulating Kir2.1-dependent macrophage polarization

doi: 10.3389/fimmu.2025.1683500

Figure Lengend Snippet: TENS induced M2 polarization of macrophages in prostate tissue. (a) Representative IHC images of CD86 + M1 macrophages and CD206 + M2 macrophages in rat prostate tissue. Scale bars: 50 μm. (b) Quantitative analyses of CD86 + M1 macrophages, CD206 + M2 macrophages, and the M1/M2 ratio in prostate tissue. CD86 + M1 and CD206 + M2 cells were counted per high-power field (HPF) at × 200 magnification. Data are shown as the mean ± SEM and were analyzed using one-way ANOVA followed by Tukey’s post-hoc test. # p < 0.05 and ## p < 0.01 vs . the control group. * p < 0.05 and ** p < 0.01 vs . the EAP group ( n = 4 per group).

Techniques: Control

Journal: Frontiers in Immunology

Article Title: TENS alleviates CP/CPPS-related inflammation and pain by modulating Kir2.1-dependent macrophage polarization

doi: 10.3389/fimmu.2025.1683500

Figure Lengend Snippet: Electrical stimulation promoted the repolarization of M1 macrophages into M2 macrophages. (a) Cell viability after ES was assessed using the CCK8 assay. (b) Flow cytometry analysis of CD86 + M1 and CD206 + M2 macrophages in control, LPS-treated, and ES-treated (0.1 V/cm, 0.25 V/cm) RAW264.7 cells. MFI, mean fluorescence intensity. (c) Reactive oxygen species (ROS) detection in control, LPS-treated, and ES-treated (0.1 V/cm, 0.25 V/cm) RAW264.7 cells. (d) Western blot analysis of M1 markers (CD86, iNOS, TLR4) and M2 markers (CD206, CD163, CD209) in control, LPS-stimulated, and LPS + ES-treated (0.25 V/cm) RAW264.7 cells. (e) Quantitative analyses of macrophage phenotype marker expression. (f) ELISA analyses of TNF-α, IL-1β, IL-6, and IL-10 secretion in macrophage culture supernatants. Data are shown as the mean ± SEM and were analyzed using one-way ANOVA followed by Tukey’s post-hoc test . ## p < 0.01 and ### p < 0.001 vs . the control group. * p < 0.05, ** p < 0.01, and *** p < 0.001 vs . the LPS group ( n = 3 per group).

Techniques: CCK-8 Assay, Flow Cytometry, Control, Fluorescence, Western Blot, Marker, Expressing, Enzyme-linked Immunosorbent Assay

Journal: Frontiers in Immunology

Article Title: TENS alleviates CP/CPPS-related inflammation and pain by modulating Kir2.1-dependent macrophage polarization

doi: 10.3389/fimmu.2025.1683500

Figure Lengend Snippet: Repolarization mechanism of ES effect in macrophages. (a) GO classification of DEPs between the LPS and ES groups related to cellular component, molecular function, and biological process categories. (b) Heatmap of differentially clustered ion transmembrane transporter activity. The color scale represents the Z -score of normalized protein abundance; red indicates upregulation, and blue indicates downregulation. (c) Representative WB images of Kir2.1 and TRPV2 expression. (d) Quantitative analyses of Kir2.1 and TRPV2 marker expression. (e) Representative IHC images of Kir2.1 in rat prostate tissue. Scale bars: 50 μm. (f) Quantitative analyses of CD86 + and CD206 + expression in macrophages measured by flow cytometry. (g) Immunofluorescence staining images of cellular membrane potential in macrophages (scale bars: 20 μm). (h) Quantitative analysis of cellular membrane potential in macrophages measured by flow cytometry. (i) Immunofluorescence staining images of intracellular Ca 2+ concentration in macrophages (scale bars: 20 μm). (j) Quantitative analysis of intracellular Ca 2+ concentration in macrophages measured by flow cytometry. MFI, mean fluorescence intensity. (k) Representative WB images of NF-κB/STAT1/STAT6 signaling pathway expression. (l) Quantitative analyses of NF-κB/STAT1/STAT6 signaling pathway expression. Data are shown as the mean ± SEM and were analyzed using one-way ANOVA followed by Tukey’s post-hoc test. ## p < 0.01 and ### p < 0.001 vs . the LPS group. * p < 0.05, ** p < 0.01, and *** p < 0.001 vs . the ES group ( n = 3 per group).

Techniques: Activity Assay, Quantitative Proteomics, Expressing, Marker, Flow Cytometry, Immunofluorescence, Staining, Membrane, Concentration Assay, Fluorescence

The regulatory effects of CaHA/PLGA microspheres on macrophages and ADSCs in vitro. (A, B) CLSM images and RFI of CD86 and CD206 expression in RAW264.7 cells co-cultured with microspheres for 2 days (n = 3). (C–F) Relative mRNA expression levels of inflammation-related genes TNF-α, IL-6, TGF-β1, and FGF-2 in RAW264.7 cells (n = 3). (G, H) Sirius red staining images and quantitative analysis (n = 3) of collagen deposition of ADSCs co-cultured with CaHA/PLGA microspheres for 3 and 7 days. (I–K) Relative mRNA expression levels of TGF-β1, FGF-2, and PDGF-A in ADSCs after 3 and 7 days of co-culture with CaHA/PLGA microspheres (n = 3). ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001; ∗∗∗∗ p < 0.0001; ns , not significant.

Journal: Bioactive Materials

Article Title: Mossy-textured hydroxyapatite-modified poly (lactic-co-glycolic acid) microspheres promote collagen regeneration via calcium/TGF-β and chemokine signaling pathways in soft tissue augmentation

doi: 10.1016/j.bioactmat.2025.12.028

Figure Lengend Snippet: The regulatory effects of CaHA/PLGA microspheres on macrophages and ADSCs in vitro. (A, B) CLSM images and RFI of CD86 and CD206 expression in RAW264.7 cells co-cultured with microspheres for 2 days (n = 3). (C–F) Relative mRNA expression levels of inflammation-related genes TNF-α, IL-6, TGF-β1, and FGF-2 in RAW264.7 cells (n = 3). (G, H) Sirius red staining images and quantitative analysis (n = 3) of collagen deposition of ADSCs co-cultured with CaHA/PLGA microspheres for 3 and 7 days. (I–K) Relative mRNA expression levels of TGF-β1, FGF-2, and PDGF-A in ADSCs after 3 and 7 days of co-culture with CaHA/PLGA microspheres (n = 3). ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001; ∗∗∗∗ p < 0.0001; ns , not significant.

Article Snippet: CD86 polyclonal antibody (1:200, 13395-1-AP, Proteintech), CD206 (1:200, 18704-1-AP, Proteintech), and TNF-α recombinant antibody (1:200, 80258-6-RR, Proteintech) were used as primary antibodies, incubated overnight at 4 °C.

Techniques: In Vitro, Expressing, Cell Culture, Staining, Co-Culture Assay

Evaluation of soft tissue filling and inflammatory response in rats. (A, B) Schematic diagram of the soft tissue filling experiment and injection sites, with at least 2 cm spacing between sites. (C) Photographs of subcutaneous soft tissue filling at 2, 4, 8, and 12 weeks. The white circles indicate the soft tissue filling areas. (D) H&E staining of the filled sites. (E, F) Immunofluorescence images and RFI of TNF-α and TGF-β expression at 2 weeks post-filling (n = 6). (G, H) Immunofluorescence images and RFI of CD86 and CD206 expression at 2 weeks post-filling (n = 6). ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001; ∗∗∗∗ p < 0.0001; ns , not significant.

Journal: Bioactive Materials

doi: 10.1016/j.bioactmat.2025.12.028

Figure Lengend Snippet: Evaluation of soft tissue filling and inflammatory response in rats. (A, B) Schematic diagram of the soft tissue filling experiment and injection sites, with at least 2 cm spacing between sites. (C) Photographs of subcutaneous soft tissue filling at 2, 4, 8, and 12 weeks. The white circles indicate the soft tissue filling areas. (D) H&E staining of the filled sites. (E, F) Immunofluorescence images and RFI of TNF-α and TGF-β expression at 2 weeks post-filling (n = 6). (G, H) Immunofluorescence images and RFI of CD86 and CD206 expression at 2 weeks post-filling (n = 6). ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001; ∗∗∗∗ p < 0.0001; ns , not significant.

Techniques: Injection, Staining, Immunofluorescence, Expressing

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